Facts-enriched characterization of cell populations. Morphological information and facts from illustrations or photos can insert on the richness of flow cytometry data. Such as, the determine shows an in any other case standard apoptosis assay applying Annexin V and PI, adding mobile imaging to characterize cells in Just about every populace to expose morphologically distinctive features. These insights couldn't are actually received from multiplex staining by itself.
As samples are obtained on the Attune CytPix Flow Cytometer, the significant-pace brightfield camera captures and outlets visuals of detected functions at a charge approximately six,000 photographs per next, depending upon the flow fee and picture size. For better flexibility, Attune Cytometric Software permits you to modify the impression capture frequency as required and choose a selected gate for image seize, if required.
Blend acoustic concentrating technology with high-parameter Investigation for adaptable spectral unmixing and traditional workflows.
Explore new insights into your experimental information by visualizing populations applying put together fluorescence and morphology info. Our software program interface wizards empower downsampling, concatenation (merged information) and dimensionality equipment which might be constructed in to the computer software for an intuitive, streamlined details analysis working experience.
"A picture isn't really very really worth one thousand dots, nonetheless it genuinely helps you to see pictures. It is great to discover illustrations or photos that correlate with staining. As an example, AnnexinV-positive cells which have been morphologically distinctive than nutritious cells, or CD14+ monocytes which might be larger sized plus more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a highly trained consumer has gated singlets confidently. Following evaluating the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of more than four% aggregates.
Optimizing gating tactic. Even sturdy manual singlet gating is error-vulnerable and continues to be a subjective decision level in almost all flow cytometry assays. Imaging can be used to verify and regulate gates to include only solitary cells of fascination.
Regular impression quality even at high throughput Acoustic focusing and a high-pace camera combine to graphic these T cells regularly at low or significant flow premiums. Very easily change concentrate and digicam configurations to fulfill experimental specifications.
Early signs of this contamination had at first been dismissed as debris. Backgating to find out new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also helps you to use morphological attributes to find exciting subpopulations that could not be evident from flow cytometry facts alone.
End users website can look at party photographs in true-time to verify sample high-quality, enhance protocols, and file the morphology of each mobile inhabitants recognized for Examination. Imaging seize also Gains from acoustic concentrating, which helps to place the cells in order that a sharp, centered image is acquired.
AI-driven automated impression Assessment While using the Attune Cytometric Program translates function features into distinctive morphology parameters that can be coupled with regular fluorescence and scatter parameters to reveal really reproducible inhabitants modifications and unique subsets.
We Formerly demonstrated the power of imaging Automobile-T/Ramos mobile interactions. Enable’s look at just the population of greatest interest, the double constructive situations, to learn more. We can now use extended morphological parameters (listed here, circularity and skewness of intensity) to further take a look at the attributes of these populations and refine gating on these occasions, escalating data robustness.
Designed-in dimensionality reduction applications amplified resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to verify predicted populace resolution in lysed entire human blood. User can perspective occasions from Each individual cluster for visual confirmation of cell phenotypes.
No wash/no lyse leukocyte Evaluation with imaging. Cells have been acquired from 24-hour-previous blood by dilution in 1 mM EDTA ( Skilled UX/UI allows buyers to prepare AI designs for expanded apps. (A) The “Practice Design” characteristic presents annotation applications and real-time product coaching accuracy.
Gating on these extended parameters permits you to immediately and accurately establish populations of curiosity and ensure gating tactic with little or no handbook critique. The Attune software program presents use of state-of-the-art equipment Discovering Investigation instruments in just an expertly made consumer interface, rendering it extremely user friendly.
The Attune Xenith cytometer is often a large-parameter spectral analyzer, giving both equally spectral unmixing and standard flow abilities for extensive and versatile cell Evaluation.
The Attune CytPix Flow Cytometer enables buyers to visualise samples for the duration of acquisition, verify the precision of gating tactics, and assistance success with dependable documentation for publication functions.
Right here, we exhibit that by gating procedures applying impression-centered quantitative parameters can distinguish interacting cells from coincident occasions to a lot more properly assess interacting cells.